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Oxidative stress thesis

Ph. D. thesis. STUDY OF OXIDATIVE STRESS AND THE ANTIOXIDANT DEFENSE. SYSTEM AFTER HEAVY METAL TREATMENT IN CARP AND IN. STREPTOZOTOCIN-INDUCED DIABETIC RATS. Zsanett Jancsó. Supervisor Edit Hermesz Ph. D. associate professor. Ph. D. School in Biology. University of Szeged. Descriptive essay writing worksheets case study research design and methods vol. Write your fully legal Will online right now Executors. Step-by-step solutions gallagher college hugh writing essay admission. Our classroom magazines help millions of children awaken a love of. My Childhood Dreams Essay - essay oxidative stress phd thesis ku for scholarships: descriptive essay my mother, essay on loss of biodiversity Free Dreams papers, essays, and research. First, I want to share some of my favorite Thanksgiving. Argumentative Essay Writing, Argument Essay Tips and Examples.

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Protein markers of oxidative stress damage Applications to experimental research and effects of fatty acids Trygve Danielsen Master Thesis in Toxicology Biochemistry, Genetics and Molecular Biology » "Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants", book edited by José A. Morales-González, ISBN 978-9-8, Published: May 22, 2013 under CC BY 3.0 license. Unidad de Genética de la Nutrición, Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México/ Instituto Nacional de Pediatría, México Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects of insulin action, insulin secretion or both [1]. Diabetes has taken place as one of the most important diseases worldwide, reaching epidemic proportions. Global estimates predict that the proportion of adult population with diabetes will increase 69% for the year 2030 [2]. Hyperglycemia in the course of diabetes usually leads to the development of microvascular complications, and diabetic patients are more prone to accelerated atherosclerotic macrovascular disease.

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Oxidative stress thesis

Electronic Thesis and Dissertation Repository. January 2017. The Association Between Oxidative Stress, Cellular. Differentiation And Galectins In Human. Promyelocytic Leukemia Cells HL-60. James R. Vinnai. The University of Western Ontario. Supervisor. Alexander Timoshenko. The University of Western Ontario. The keystone aquatic organism Daphnia magna is extensively used to assess the toxicity of chemicals. This has recently lead to an increase in the omics literature focusing on daphnids, an increase fuelled by the sequencing of the Daphnia pulex genome. Yet, no omics study has looked directly at oxidative stress (OS) in daphnids, even though OS is of primary importance in the response of aquatic organisms to their changing environment and is often induced by anthropogenic xenobiotics. This thesis thus focuses on the application of redox-proteomics, the study of the oxidative modification of proteins, to D. magna Specifically, daphnids were exposed to copper or paraquat, two well studied prooxidants, and protein carbonyls were labelled with fluorescein-5-thiosemicarbazide prior to twodimensional electrophoresis (2DE). This showed clearly that both compounds affect a different portion of the proteome. The identified proteins indicated that energy metabolism was affected by paraquat, while copper induced a reduction of the heat shock response (heat shock proteins, proteases and chaperones) a counterintuitive result which may be adaptative to metal toxicity in arthropods. The same approach was then applied to the study of the toxicity mechanism of silver nanoparticles (Ag NP), an increasingly utilised form of silver with expected environmental toxicity, and its comparison to silver nitrate.

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Oxidative stress thesis

The role of dietary antioxidants in exercise-induced oxidative stress and athletic. The two broad themes of the thesis are the effect of usual dietary intake on. Section of Dermatology, Department of Surgery and Translational Medicine, University of Florence, Ospedale Piero Palagi, Viale Michelangelo 41, 50125 Florence, Italy Received 24 December 2014; Accepted 2 March 2015Academic Editor: Thomas Kietzmann Copyright © 2015 Roberta Colucci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Vitiligo is an acquired dermatological disease frequently associated with autoimmune thyroid disorders. Several theories have been proposed so far to unravel the complex vitiligo pathogenesis. Currently, the autocytotoxic and the autoimmune theories are the most accredited hypothesis, since they are sustained by several important clinical and experimental evidences. A growing body of evidences shows that autoimmunity and oxidative stress strictly interact to finally determine melanocyte loss. In this scenario, associated thyroid autoimmunity might play an active and important role in triggering and maintaining the depigmentation process of vitiligo. Vitiligo is an acquired dermatological disorder characterized by circumscribed depigmented macules due to the loss of functional melanocytes in the epidermis [1].

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Oxidative stress thesis

Publications related to the thesis I. Olah Z, Pakaski M, Janka Z, Kalman J Marking the markers1.4. Oxidative stress The oxidative stress is one of the earliest process in the course of AD development. WHITE MATTER HYPERINTENSITY VOLUME AS A BIOMARKER OF OXIDATIVE STRESS IN ACUTE ISCHEMIC STROKE Zachary A. Rost, MGH Stroke Service, Department of Neurology, Massachusetts General Hospital; Jeffrey B. Milbury, Antioxidant Research Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA; Michael K. Parides, Department of Health Evidence and Policy, Mount Sinai School of Medicine, New York, NY; and Karen L. Furie, MGH Stroke Service, Department of Neurology, Massachusetts General Hospital (Sponsored by Walter N. Kernan, Section of General Internal Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT). Oxidative stress contributes to brain injury during ischemic stroke, but antioxidant clinical trials have been unable to demonstrate a benefit to date. The all comers approach to patient selection in these trials may have contributed to their lack of success. We hypothesize that white matter hyperintensity is a biomarker that can identify ischemic stroke patients with increased levels of oxidative stress and improve future trials. White matter hyperintensity represents chronic loss of cellular brain tissue and has been correlated with oxidative stress in humans and animal models.

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Oxidative stress thesis

This thesis is based on the following publications, referred to in the text by their Roman numerals I. Koivula*, M. J. and Eeva, T. 2010 Metal-related oxidative stress in birds. Environmental Pollution, 158, 2359-2370. II. Koivula*, M. J. Kanerva, M. Salminen, J.-P. Nikinmaa, M. and Eeva, T. 2011. Metal pollution indirectly. This study aimed at evaluating the oxidative stress in mitochondria isolated from the brain and liver of mice treated with riparin A, as well as the locomotor activity and myorelaxant effect of this compound. Behavioral models of rota rod and open field tests were used for locomotor activity and myorelaxant effect evaluation. The animals were divided into five groups (n = 8), which were treated with: diazepam (1 mg/kg, i.p), riparin A (5, 10, and 20 mg/kg, o.r.) or vehicle (0.9% saline, o.r.). The oxidative stress evaluation was carried out in mitochondria isolated from the brain and liver of mice from five experimental groups (n = 8), which were treated with: ascorbic acid (250 mg/kg; positive control), vehicle (0.9% saline; negative control) and riparin A (5, 10 and 20 mg/kg).

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Oxidative stress thesis

School of Doctoral Studies in Biological Sciences. University of South Bohemia in České Budějovice. Faculty of Science. The role of insect adipokinetic hormones in oxidative stress. Ph. D. Thesis. RNDr. Josef Večeřa. Supervisor Prof. RNDr. Dalibor Kodrík, CSc. Biology Centre ASCR, Institute of Entomology. Laboratory of. The Effects of Antidepressant Treatment on Oxidative Stress and Anti-oxidants in Subjects with Depression INTRODUCTION Depression is a common disorder characterized by persistent feelings of hopelessness and sadness. According to the DSM-IV criteria, the 2 core symptoms are a depressed mood and anhedonia (reduced pleasure and loss of interest in activities). It was pointed out by the NEMESIS-2 research that the prevalence of mood disorders in the age range between 18 and 65 is about 4.9% and 7.4% per year among men and women, respectively (De Graaf, Ten Have & Dorsselaer, 2010). Major depressive disorder (MDD) is the most common mood disorder and has been accepted to be an independent risk factor for many illnesses, such as cardiovascular disease (Loomba, 2015). Cuijpers & Smit (2002) observed that depression is also accompanied by a higher mortality rate. Furthermore, depression has a profound negative influence on quality of life and functioning; it accounts for 8.2% of global years lived with disability (YLDs) and for 2.5% of global disability adjusted life years (DALYs). This makes depression a huge global health priority, which is worldwide the second leading cause of disability and sickness absence (Ferrari et al., 2013). Oxidative stress has been implicated in the pathophysiology of depression (Pandya, Howell, & Pillai, 2012). Oxidative stress is a metabolic condition in which the amount of reactive oxygen species (ROS) is increased or the quantity of anti-oxidants is decreased.

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Oxidative stress thesis

Sep 23, 2014. Effects of propofol and N-acetylcystein on oxidative stress in a dog model. Doctoral thesis, Faculty of Science Department of Biology. Packaging technology allows for 100% resin usability. Unique benefits available only to Pürblack Live Resin. You can typically find them in pill and powder form. Shilajit is a substance that has been used in Eastern European Holistic and Ayurvedic medicine, an ancient practice that spans over 5,000 years. Passes all authenticity tests for Pürblack Live Resin, Shilajit and Mumie. Highest level of protection against counterfeits from India and Russia. The resin can be rolled into pills for fast swallowing. All benefits unique to high-end genuine mumie resins and powders. Appearances aside, the aforementioned substance, shilajit, comes with a medley of health benefits. Certificate of Analysis and Independent testing are public. Each jar has a permanent individual serial number matching the content. All benefits unique to high-end genuine shilajit resins and powders. Highly outperforms all genuine shilajit and mumie in quality and efficacy. Produced under American patent – pending technology. However, what if I told you that there’s a supplement that is black and tar-like? Eastern European holistic medicine along with Ayurveda originated in the east of Asia. Both practices were used primarily to improve health. To this day, many Asian countries still follow these ancient practices. If one thing can be said about traditional health practices, they stood the test of time. All holistic health practices have been built upon the concept that maintaining equilibrium in the body.

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Oxidative stress thesis

Oxidative stress reflects an imbalance between the systemic manifestation of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage. This thesis addressed the sensory functions of the gastrointestinal (GI) tract with a focus on the effect of oxidative stress on afferent nerve activity from small intestine and colon in young and aged mouse. Oxidative stress appears to be involved in the pathogenesis of many gastrointestinal conditions, such as inflammatory bowel disease (IBD), colon cancer, and may contribute to the gut dysfunction in ageing. How diverse regions of the gut react to, and handle, elevated levels of ROS in young and aged is unclear. Here, I investigated the effect of oxidative stress on afferent nerve activity in young and aged mice, and if it contributes to age associated changes. The study used in vitro afferent nerve recordings from jejunum and colon of male mice and concluded that colonic sensory neurons are more sensitive to oxidative stress than jejunum sensory neurons. In the aged group, decreased afferent mechanosensitivity associated with a greater oxidative status was observed only in the aged colonic mucosa. Findings obtained by RNA microarray analysis suggested that the difference between the mouse jejunum and colon in ROS production genes and secondary antioxidant genes may have contributed to the colonic afferent being more sensitive to oxidative stress. In addition, upregulation of inflammatory related genes associated with long-life exposure to high endogenous ROS level are possible factors for colon being more inclined to develop diseases or decreased function as a result of normal ageing.

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Oxidative stress thesis

Oxidative stress Dhalla et al 1994, 1996, Hill et al 1996. Excessive ROS may directly induce cellular injury via oxidation of DNA, lipids, and proteins associated with cell death, Condensed tannins (proanthocyanidins, polyflavonoid tannins, catechol-type tannins, pyrocatecollic type tannins, non-hydrolyzable tannins or flavolans) are polymers formed by the condensation of flavans. They are called proanthocyanidins as they yield anthocyanidins when depolymerized under oxidative conditions. Different types of condensed tannins exist, such as the procyanidins, propelargonidins, prodelphinidins, profisetinidins, proteracacinidins, proguibourtinidins or prorobinetidins. All of the above are formed from flavan-3-ols, but flavan-3,4-diols, called (leucoanthocyanidin) also form condensed tannin oligomers, e.g. leuco-fisetinidin form profisetinidin, and flavan-4-ols form condensed tannins, e.g. 3',4',5,7-flavan-4-ol form proluteolinidin (luteoforolor). One particular type of condensed tannin, found in grape, are procyanidins, which are polymers of 2 to 50 (or more) Catechin units joined by carbon-carbon bonds. These are not susceptible to being cleaved by hydrolysis. While many hydrolyzable tannins and most condensed tannins are water-soluble, several tannins are also highly octanol-soluble. Pycnogenol is a dietary supplement derived from extracts from maritime pine bark, is standardised to contain 70% procyanidin and is marketed with claims it can treat many conditions; however, according to a 2012 Cochrane review, the evidence is insufficient to support its use for the treatment of any chronic disorder.

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Oxidative stress thesis

Aug 1, 2018. PDF The main goal of this thesis project has been to investigate the role of the oxidative stress OS in metal-induced toxicity and to test. Metabolic syndrome (Met S) can classically be defined by physiological factors, clinical factors, and metabolic factors that are responsible for increased risk of cardiovascular diseases (CVD) and type 2 diabetes (T2D). The International Diabetes Federation has released the most recent definition of Met S, defined by increased waist circumference and two of the following factors: increased serum triglycerides or decreased HDL-cholesterol, increased fasting blood glucose, or hypertension. Met S is also profoundly defined by chronic low-grade inflammation. This proinflammatory state can develop as a result adipocyte hypertrophy and hyperplasia that is responsible for increased secretion of glycerol, free fatty acids, and secretion of adipocytokines. States of adipose tissue dysregulation due to obesity can directly affect systemic inflammation, and the progression to diseased states of insulin resistance and Met S. Adipose tissue does not only contribute to the pathophysiology through proinflammatory signaling, but can alter systemic lipid content by releasing circulating free fatty acids (FFAs) that accumulate in liver, induce insulin resistance in skeletal muscle, and impair pancreatic β-cell functions, all contributing to the potential development of severe metabolic diseases. Most importantly though, oxidative stress has been suggested to be an early event in the pathophysiology and manifestation of chronic metabolic disorders in humans including Met S, T2D, coronary artery disease, and hypertension. The excess storage of lipids in white adipose tissue (WAT), known as obesity, results in oxidative stress and infiltration of macrophages that can alter WAT metabolism.

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Oxidative stress thesis

Protein markers of oxidative stress damage Applications to experimental research and effects of fatty acids. Trygve Danielsen. Master Thesis in Toxicology. Department of Biosciences. Faculty of Mathematics and Natural Sciences. University of Oslo. April 2015. Traditional medicines worldwide have long used herbal remedies for treating a wide range of infections, ailments and diseases. That’s because many of them — especially taken as tea — contain natural compounds with some serious health benefits…Take pu-erh, for example, the tea that helps you drop pounds, cholesterol points and blood sugar levels. Like most well-known herbal teas, pu-erh originates from China. But an unusual tea you’ve likely never heard of, from a different part of the world, may be stealing the show soon…Originating in South Africa, rooibos tea (also known as red tea or red bush tea) is a caffeine-free beverage that comes from the shrub. The leaves of the shrub are fermented to create an herbal tea that makes the perfect substitute for regular caffeinated tea.

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Oxidative stress thesis

Using this method, the individual oxidative stress-related exposures are combined such that higher OBS values reflect the relative predominance of anti-oxidant factors. The primary objective of this dissertation is to examine the association between OBS and human degenerative diseases and mortality. In the first study. The objectives of this study were to investigate the effects of anesthesia with isoflurane or propofol with addition of N-acetylcysteine during open and laparoscopic ovariectomy in bitches on malondialdehyde concentration, total oxidative status and antioxidant capacity. Also, the aim was to investigate possible synergistic effects of propofol and N-acetylcysteine. The study was prospective, randomised and controlled. 61 female dogs were allocated in 6 groups according to surgical technic and type of anesthesia. Blood samples for the analysis were taken after the induction of anesthesia, at the end of the procedure and 24 hours after. High performance liquid chromatography and colorimetric, enzymatic tests were used for the analysis. In most of the groups statistically significant differences in parameters of oxidative stress were not found. However, analysis of statistical differences which were found and observation of positive trends during perioperative period, discerned trend that laparoscopic surgery and anestehsia with propofol could have beneficial effects.

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Oxidative stress thesis

Oxidative stress in sperm competition games Experimental tests of the soma vs. germline allocation trade-off in wild House Sparrows Passer domesticus. Thesis. Before you read this post, realize that my bad experience and other people’s negative experiences are meant to give you pause. Ultimately, you will have to experiment for yourself and see how Piracetam works for you. Just be skeptical of all of the positive and negative reviews. The risk of permanent harm of taking a low dose of piracetam a few times is extremely small, so experimenting with it is in my opinion a worthwhile risk. The purpose of this post is merely to show that there are some people who do not thrive off of it, but again I highly doubt that there are any life threatening or permanent side effects. I have also written a post on the benefits to counteract some of these negative reports: I am asked often why I don’t supplement with the racetams – specifically piracetam. Piracetam, for those who don’t know, is the most popular smart drug or nootropic. When I experimented with piracetam, I temporarily experienced pretty bad brain fog from it and my IQ must have transiently dropped quite a bit. I tried 2 different and reliable brands of piracetam (nootropil, cognitive nutrition), as well as aniracetam and oxiracetam. I varied the dosages of piracetam and choline, as well as the types of choline.

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Oxidative stress thesis

ESTROGEN'S EFFECTS ON OXIDATIVE STRESS LEVELS IN GLIAL. CELLS. By. Jacob J. Dirkman. A Thesis Submitted to the. HONORS COLLEGE. In Partial. ◉ Black Acetic Acid ◉ As a natural organic acid, the Black Acetic Acid of Theraderm Black Peel is made through the fermentation of black rice. It contains large amounts of organic materials, minerals and especially high concentrations of essential amino acids. Recent researches on black vinegar have revealed its anticancer properties in vivo on rats and in vitro on human cancer cells and as well as antioxidant activity by reducing oxidative stress: J Nutr Health Aging. ◉ KI (Potassium Iodide) ◉ Empirically used for deep fungal infection and severe vasculities induced wound In vivo and vitro study showed fast wound recovery effects of KI. ◉ Jasmonic Acid & Salicylic Acid ◉ Jasmonic Acid and Salicylic Acid are secreted by plants to defend themselves when they are attacked or damaged. Both Jasmonic Acid and Salicylic Acid play an important role in the pathogen defence response and also have the antiflammatory effect as well as the exfoliating property. Jasmonic Acid is a good alternate of Salicylic Acid.

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Oxidative stress thesis

The cell envelope is a permeability barrier that is essential for the viability of Gram-negative bacteria and protects them against toxic compounds, including. White matter lesions (WML), identified as hyperintensities on T2-weighted magnetic resonance images (MRI) in the ageing brain, are associated with dementia and depression in the elderly. Ischaemia may contribute to their pathogenesis but the exact role of glial cell pathology remains unclear. Recent studies have concluded that oxidative stress is present in high levels in the deep subcortical white matter lesions when compared to periventricular white matter lesions. The current study investigates the hypothesis that oxidative DNA damage contributes to the pathogenesis of WML, specifically the deep subcortical WM (DSCL). Oxidative DNA damage was investigated in WML and control WM, both from cases with WML (referred to as lesional controls) and without WML derived from the MRC-Cognitive Function and Ageing Study. Lesions were previously identified using post mortem MRI. 8-hydroxy-2’-deoxyguanosine (8-OHd G) was detected by immunohistochemisty and nuclear expression quantified. Double staining was performed to colocalise 8-OHd G with markers for specific cell type (e.g. Expression of Malonaldehyde (MDA) (marker of lipid peroxidation), gamma histone H2AX (ɣH2AX) and DNA dependent protein kinase (DNA-PK) (markers of DNA damage response) were quantified by Western Blotting.

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Oxidative stress thesis

MECHANISMS OF H2O2-INDUCED OXIDATIVE STRESS IN ENDOTHELIAL CELLS by Christian Hannon Coyle An Abstract Of a thesis submitted in partial fulfillment of the requirements for the Doctor of Also see: Bowel Toxins Accelerate Aging Ray Peat, Ph D on the Benefits of the Raw Carrot Protective Cascara Sagrada and Emodin Fermentable Carbohydrates, Anxiety, Aggression Protective Bamboo Shoots Endotoxin-lipoprotein Hypothesis Endotoxin: Poisoning from the Inside Out Protection from Endotoxin How does estrogen enhance endotoxin toxicity? Estrogen, Endotoxin, and Alcohol-Induced Liver Injury Alcohol Consumption – Estrogen and Progesterone In Women Autoimmunity and Intestinal Flora Hypothyroidism, Intestinal Bacterial Overgrowth, & Lactose Intolerance Estrogen, Endotoxin, and Alcohol-Induced Liver Injury Protective “Essential Fatty Acid Deficiency” PUFA and Liver Toxicity; Protection by Saturated Fats Can Endurance Sports Really Cause Harm? The Lipopolysaccharides of Endotoxemia and Their Effect on the Heart “The maladaptive sequence, starting from stress or hypothyroidism, would typically involve increased absorption of endotoxin, leading to interference with mitochondrial respiration, a shift to fat oxidation, inflammation, and the increase of a wide range of stress hormones. Each of these happens to interfere with the production of progesterone, leading to increased LH.” “The liver is the major source of the acute phase proteins, and it is constantly burdened by toxins absorbed from the bowel; disinfection of the bowel is known to accelerate recovery from stress.” “It takes a few days for the intestine to adjust to raw carrot, but the indigestible fiber is very protective for the intestine. Boiled bamboo shoots, which are also mostly indigestible, have a similar effect. These fibers prevent the reabsorption of estrogen in the intestine, and can shift the balance away from cortisol and estrogen, toward progesterone and thyroid, in just a few days of regular use.

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Oxidative stress thesis

Oxidative stress and exercise training Effects of exercise intensity, training status, sex, hydration and heat stress Thesis, Master of Physical Education. The Daily Slot Challenge runs every day from AM to PM. All wins on slot games will contribute to your leaderboard score. The top 3 players at the end of the day will receive virtual credit prizes. The more you win the higher your score so get playing to become the Daily Slot Challenge Champion!

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Background & Objectives Oxidative stress has been investigated to explain various physiological as well as pathological basis of many medical conditions. But very few data concerning the Background: Cells continuously produce free radicals (FR) and reactive oxygen species (ROS) as part of metabolic processes. These free radicals are neutralized by an elaborate antioxidant defence system consisting of enzymes. An imbalance between ROS / FR and antioxidants is often referred to as oxidative stress and is involved in the pathogenesis of various disorders including cardiovascular disease, neurodegenerative diseases, diabetes and several types of cancer (Campbell et al. Selenium (Se), as part of the glutathione peroxidase (GPx) family of enzymes, has the ability to detoxify these ROS / FR, increasing protection against this stress. Regular training has been shown to increase antioxidant capacity; it is through adaptation that increased antioxidant capacity may alleviate oxidative stress. Objectives: The present investigation is designed to test the hypothesis that Se supplementation and / or regular High Intensity Interval Training (HIIT) can alleviate exercise - induced oxidative stress following a single bout of HIIT. Method: Twenty-two healthy female participants, who participated in intermittent sports, were recruited. In a randomised manner, participants were equally allocated to either a Se only group (250 ug sodium selenite/day) or a Se HIIT group (250 ug sodium selenite / day 2 sessions HIIT / week) for 3 weeks. Measures of fitness, malondialdehyde (MDA), glutathione peroxidase (GPx) in plasma and red blood cells (RBC) and total antioxidant capacity (TAC) were assessed before and after an initial (baseline) and last bout of HIIT. Results: No statistically significant changes were identified for any of the measured markers of oxidative stress or antioxidant capacity, although a number of trends toward altered activity were noted.

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